Interspecies hybridizations in situ with bovine heterosome painting probes for identification of sex chromosomes in fallow deer (Dama dama)
نویسندگان
چکیده
The phenomenon of syntenic genetic conservation of chromosomes enables application of molecular probes obtained from one species of animals to detect homologous DNA segments in another species. The aim of this study was identification of sex chromosomes in fallow deer (Dama dama), using in interspecies hybridizations in situ (Zoo-FISH technique) the bovine heterosomes painting probes. The results obtained showed distinct yellow-green signals in big acrocentric chromosomes X and strong red fluorescence signals in small submetacentric heterosomes Y in all fallow deer metaphase plates. The studies confirmed the high degree of genetic conservation of heterosome synteny groups in the species belonging to Ruminantia, which makes it possible to use of bovine, heterologous sex chromosomes painting probes in interspecies comparative, phylogenetic and evolutionary analyses.
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